| 生物体内泛醌的种类及合成条件的探讨 |
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| 引用本文： | 朱旭芬,曾云中,吴雪昌.生物体内泛醌的种类及合成条件的探讨[J].浙江大学学报(理学版),2000,27(3):324-328. |
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| 作者姓名： | 朱旭芬 曾云中 吴雪昌 |
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| 作者单位： | 浙江大学生命科学学院!浙江杭州310012 |
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| 基金项目： | 浙江省留学回国基金，国家教委留学回国人员科研 !(G59971)资助项目 |
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| 摘 要： | 通过薄层层析、高效液相色谱等方法对大肠杆菌、醇洒酵母、栗酒裂殖酵母和烟草等四种不同生物所含的泛醌（即辅酶Ｑ）进行了定性分析，确定了各种生物所含的泛醌种类，并利用表达泛醌合成基因的大肠杆菌培养过程澡泛醌含量的变化以及添加前体物质对提高大肠杆菌泛醌合成量进行了初步研究，确定了稳定生长前中期是生物体中泛醌含量最高时期引进泛醌合成的外源基因以及在培养其中添加０．１ｍＭ前体物质一对羟甲酸可以提高生物体内泛醌
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| 关 键 词： | 泛醌 高效液相色谱 生物体 种类 合成条件 辅酶Q |
Study on ubiquinone species and synthetic condition for organisms. |
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| ZHU Xu-fen,ZENG Yun\|zhong,WU Xue\|chang.Study on ubiquinone species and synthetic condition for organisms.[J].Journal of Zhejiang University(Sciences Edition),2000,27(3):324-328. |
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| Authors: | ZHU Xu-fen ZENG Yun\|zhong WU Xue\|chang |
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| Abstract: | Ubiquinone, extracted from \$Escherichia coli, Saccharomyces cerevisiae, Schizosaccharomyces pombe\$ and \$Nicotiana tabacum\$, were analyzed by thin\|layer chromatography (TLC) and high performance liquid chromatography (HPLC), and the ubiquinone species of them were confirmed. Then, ubiquinone biosynthetic genes were expressed in \$E. coli\$. Effect of cultivative time and precursor \$p\$\|hydroxybenzoate on ubiquinone yield was investigated. The results show that the higher level of ubiquinone is produced in the stationary phage of growth period, and over\|expressing exogenous genes as well as adding 0.1mM \$p\$\|hydroxybenzoate may increase ubiquinone yield of organism. |
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| Keywords: | ubiquinone precursor HPLC |
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