Fractionation of polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans and planar polychlorinated biphenyls by high performance liquid chromatography on a pyrenyl-silica column |
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Authors: | M Martínez-Cored E Pujadas J Díaz-Ferrero M Coll R Martí F Broto-Puig L Comellas M C Rodríguez-Larena |
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Institution: | (1) Environmental Laboratory, Institut Químic de Sarrià (Ramon Llull University), Via Augusta 390, E-08017 Barcelona, Spain, ES;(2) Section of Chromatography, Institut Químic de Sarrià (Ramon Llull University), Via Augusta 390, E-08017 Barcelona, Spain, ES |
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Abstract: | Analytical procedures for the determination of polychlorinated dibenzo-p-dioxins (PCDD), polychlorinated dibenzofurans (PCDF) and non-ortho polychlorinated biphenyls (PCB) require a fractionation step to separate PCDD/F from planar PCB and the bulk of PCB. An HPLC
method which achieves the separation of the bulk of PCB (0–6 mL of hexane), mono-ortho PCB (6–8 mL of hexane), non-ortho PCB (8–15 mL of hexane) and PCDD/F (15–50 mL of toluene) on a PYE column (2-(1-pyrenyl) ethyldimethylsilylated silica gel)
in a single step without the use of backflush as other authors proposed was developed. The method shows a good accuracy and
precision and it is linear in the range studied, e.g from 5.8 to 2420 pg injected in HPLC for TCDD/F, from 28.8 to 12100 pg
for PeCDD/F, HxCDD/F, HpCDD/F and from 57.6 to 24200 pg for OCDD/F. It has been successfully applied to the analysis of technical
mixtures of PCB (Aroclors), a pine wood sample and several water samples of different origins.
Received: 29 November 1998 / Revised: 25 February 1999 / Accepted: 3 March 1999 |
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