Characterization and purification of polyunsaturated fatty acids from microalgae by gas chromatography-mass spectrometry and countercurrent chromatography |
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| Authors: | O. Bousquet N. Sellier F. Le Goffic |
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| Affiliation: | (1) Laboratoire de Bioorganique et Biotechnologies, Ecole Nationale Supérieure de Chimie de Paris, 11 rue P. & M. Curie, 75231 Paris Cedex 05, France;(2) Laboratoire de Spectrométrie de Masse, Ecole Nationale Supérieure de Chimie de Paris, 11 rue P. & M. Curie, 75231 Paris Cedex 05, France |
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| Abstract: | Summary The present study was undertaken in order to characterize then to purify fatty acids from marine phytoplankton. From a crude mixture of fatty acid methyl esters it was possible to isolate by countercurrent chromatography a mixture of four polyunsaturated fatty acid methyl ester identified as being hexadecatrienoic acid methyl ester, octadecatetraenoic acid methyl ester, eicosapentaenoic acid methyl ester and docosahexaenoic acid methyl ester by gas chromatography coupled with mass spectrometry in electron impact and in positive-ion chemical ionization mode. The four polyunsaturated fatty acids are in different ratios in mixtures from the two microorganisms:Skeletonema costatum andIsochrysis galbana. |
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| Keywords: | Gas chromatography/mass spectrometry Countercurrent chromatography Polyunsaturated fatty acids Algae |
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