Isolation and structure determination of bioactive isoflavones from callus culture of Dipteryx odorata |
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Authors: | Januário Ana Helena Lourenço Miriam Verginia Domézio Lilian Aparecida Pietro Rosemeire Cristina Linhari Rodrigues Castilho Marcelo Santos Tomazela Daniela Maria da Silva Maria Fátima das Graças Fernandes Vieira Paulo Cezar Fernandes João Batista França Suzelei de Castro |
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Institution: | Unidade de Biotecnologia, Universidade de Ribeir?o Preto, UNAERP, S?o Paulo, Brazil. ajanuari@unaerp.br |
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Abstract: | Crude extracts of callus and roots of Dipteryx odorata were analyzed by HPLC to detect and quantify isoflavone contents. Based on spectroscopic and X-ray crystallography data the structures of two isoflavones were elucidated as 7-hydroxy-4',6-dimethoxyisoflavone and 3',7-dihydroxy-4',6-dimethoxyisoflavone. The production of dry biomass of 7-hydroxy-4',6-dimethoxyisoflavone in cultured callus was 4.12 mg/g, approximately eleven fold higher than the amount accumulated in roots of D. odorata wild-growing plants. The 7-hydroxy-4',6-dimethoxyisoflavone was effective against glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma cruzi. This is the first report on those bioactive isoflavones accumulated in callus of D. odorata. |
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