High throughput screening or drug impurity profiles using gradients in normal phase chromatography |
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Authors: | J. A. Blackwell L. Tang D. Xiang |
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Affiliation: | (1) Chemical Process Research and Development, Dupont Pharmaceuticals Company, Chambers Works, PRF-1, 08023 Deepwater, NJ, USA |
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Abstract: | Summary Developmental drug substances and a variety of precursors and process-related impurities were separated using mobile phase gradients under normal phase conditions. These separation conditions produced efficient separations in a fraction of the time necessary for comparable reversed-phase separations. The use of elevated temperatures, polar modifiers and moderate levels of acidic and basic additives allowed highly polar analytes to be eluted with high efficiency and selectivity using fast flow rates. Reproducibility for retention time and peak areas was demonstrated to be very good despite very short equilibration times. |
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Keywords: | Supercritical fluid chromatography Normal phase conditions High throughput screening Impurity profile |
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