Photoinduced interactions between colloidal TiO2 nanoparticles and calf thymus-DNA |
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Authors: | A. Kathiravan R. Renganathan |
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Affiliation: | School of Chemistry, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India |
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Abstract: | The interaction of colloidal TiO2 nanoparticles with calf thymus-DNA was studied by using absorption, FT-IR, steady state and time resolved fluorescence spectroscopic techniques. The apparent association constant has been deduced (Kapp = 2.85 × 103 M−1) from the absorption spectral changes of the DNA-colloidal TiO2 nanoparticles using the Benesi–Hildebrand equation. Addition of colloidal TiO2 nanoparticles quenched the fluorescence of EtBr–DNA. The number of binding sites (n = 0.97) and the apparent binding constant (K = 6.68 × 103 M−1) were calculated from relevant fluorescence quenching data. The quenching, through a static mechanism, was confirmed by time resolved fluorescence spectroscopy. |
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Keywords: | Colloidal TiO2 Fluorescence quenching DNA |
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