Biocatalytic Modification of Naturally Occurring Iron Porphyrin |
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| Authors: | Subhalakshmi Nagarajan Ramaswamy Nagarajan Rahul Tyagi Jayant Kumar Ferdinando F Bruno Lynne A Samuelson |
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| Institution: | 1. Department of Chemistry , University of Massachusetts , Lowell, MA, 01854;2. Center for Advanced Materials , University of Massachusetts , Lowell, MA, 01854;3. Plastics Engineering , University of Massachusetts , Lowell, MA, 01854;4. Institute for Nanoscale Science and Engineering , University of Massachusetts , Lowell, MA, 01854;5. Physics , University of Massachusetts , Lowell, MA, 01854;6. U.S Army Natick Soldier Research , Development and Engineering Center , Natick, MA, 01760 |
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| Abstract: | Hematin, a hydroxyferriprotoporphyrin, is the stable, oxidized form of heme. Heme has been reported to be the active catalytic center of naturally occurring peroxidases such as horseradish peroxidase (HRP). While there have been reports on the use of hematin as a catalyst for oxidative polymerization reactions, these reactions could be carried out only at high pH conditions due to limited aqueous solubility of hematin at lower pH conditions. We report here the biocatalytic modification of hematin using a lipase, Novozyme-435. Hematin has been modified by tethering monomethoxy polyethylene glycol (mPEG) chains which provide aqueous solubility over a fairly wide range of pH conditions. This pegylated Hematin (PEG-Hematin) is synthesized via a one-step solventless reaction and the products formed can be isolated with minimal purification. The PEG-Hematin synthesized serves as a robust alternative to HRP for the polymerization of aniline and phenol. |
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| Keywords: | biomimetic catalysts green chemistry hematin lipases enzymatic polymerization |
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