High efficient delivery of siRNA into tumor cells by positively charged carbon dots |
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Authors: | Jie Wang Songyan Liu Ying Chang Le Fang Kai Han |
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Affiliation: | Department of Neurology, The China-Japan Union Hospital of Jilin University, Changchun, China |
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Abstract: | AbstractNowadays development of safe and simple siRNA delivery system is still a great challenge. Carbon dots have attracted considerable attention in bioimaging, drug delivery, nanosensors, and other fields during the past few years. In this study, we successfully developed carbon dots (TEPA-CDs) as siRNA nanocarriers based on glucose and tetraethylene pentamine. TEPA-CDs have a mean diameter of less than 10?nm and positive charged decoration. TEPA-CDs could condense siRNA into stable complexes without no obvious premature release. Cellular uptake analysis clearly showed that Cy3-labeled siRNA could be uptake by HeLa cells. GFP expression in HeLa-EGFP cells could be significantly inhibited by TEPA-CDs/siRNA complexes. Our study indicated that TEPA-CDs could be used as a siRNA nanocarrier to tumor cells. |
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Keywords: | Carbon dots siRNA drug delivery gene silencing cellular uptake |
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