Biocompatibility In-vitro of Gel/HA Composite Scaffolds Containing Nano-Bioactive Glass for Tissue Engineering |
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| Authors: | Zhihua Zhou Baoli Ou Tianlong Huang Wennan Zeng Lihua Liu Qingquan Liu |
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| Institution: | 1. Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education , Hunan University of Science and Technology , Xiangtan , P. R. China;2. Hunan Province College Key Laboratory of QSAR/QSPR , Hunan University of Science and Technology , Xiangtan , P. R. China;3. School of Chemistry and Chemical Engineering , Hunan University of Science and Technology , Xiangtan , P. R. China;4. School of Chemistry and Chemical Engineering , Hunan University of Science and Technology , Xiangtan , P. R. China;5. Department of Orthopedics, the Second Xiangya Hospital , Central South University , Changsha , P. R. China |
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| Abstract: | Designing and fabricating nanocomposite scaffolds for bone regeneration from different biodegradable polymers and bioactive materials are an essential step to engineer tissues. In this study, the composite scaffold of gelatin/hyaluronic acid (Gel/HA) containing nano-bioactive glass (NBG) was prepared by using freeze-drying method. The biocompatibilities in-vitro of the Gel-HA/NBG composite scaffolds, including MTT assay, ALP activity, von Kossa staining and tetracycline staining, were investigated. The SEM observations revealed that the prepared scaffolds were porous with three-dimensional (3D) and interconnected microstructure, agglomerated NBG particles were uniformly dispersed in the matrix. MTT results indicated that the tested materials didn't show any cytotoxicity. The presence of NBG in the composite scaffold further enhanced the ALP activity in comparison with the pure Gel/HA scaffold. The von Kossa staining and tetracycline staining results also indicated that the NBG may improve the cell response. Therefore, the results indicated the nanocomposite scaffold made from Gel, HA and NBG particles could be considered as a potential bone tissue engineering implant. |
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| Keywords: | Gelatin hyaluronic acid nano-bioactive glass composite scaffold biocompatibility |
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