| TBARS法测定胰岛素口腔喷雾剂中脂质过氧化物研究 |
| |
| 引用本文： | 余灯广,高秋华,胡锦东,陈然.TBARS法测定胰岛素口腔喷雾剂中脂质过氧化物研究[J].分析科学学报,2005,21(1):78-80. |
| |
| 作者姓名： | 余灯广 高秋华 胡锦东 陈然 |
| |
| 作者单位： | 华中科技大学药物研究所,武汉,430074 |
| |
| 摘 要： | 采用硫代巴比妥酸反应物法(ThiobarbituricAcidReactiveSubstanceAssay,TBARS)对胰岛素口腔喷雾剂(InsulinBuccalSpray,IBS)中脂质过氧化程度进行了测定,并对IBS前处理方法、稀释倍数、加热作用亦进行了探究。发现在使用丙二醛(Malondialdehyde,MDA)测试盒测量IBS中脂质过氧化物(LipidPeroxide,LPO)实验中,样品应该先用有机溶剂稀释或用含0.8%(m/V)TritonX 100的0.01mol·L-1HCl溶液破乳;而乳剂测量前以稀释10倍为宜。对不同时期的IBS制剂检测,发现在IBS的一年保质期内,其脂质过氧化程度变化不大。
|
| 关 键 词： | 胰岛素口腔喷雾剂 脂质过氧化 硫代巴比妥酸反应物法 |
| 文章编号： | 1006-6144(2005)01-0078-03 |
| 修稿时间： | 2003年7月9日 |
Investigation of Lipid Peroxide in Insulin Buccal Spray by Thiobarbituric Acid Reactive Substance Assay |
| |
| YU Deng-guang,GAO Qiu-hua,HU Jin-dong,Chen Ran.Investigation of Lipid Peroxide in Insulin Buccal Spray by Thiobarbituric Acid Reactive Substance Assay[J].Journal of Analytical Science,2005,21(1):78-80. |
| |
| Authors: | YU Deng-guang GAO Qiu-hua HU Jin-dong Chen Ran |
| |
| Institution: | YU Deng-guang~*,GAO Qiu-hua,HU Jin-dong,Chen Ran |
| |
| Abstract: | The contents of lipid peroxide (LPO) in insulin ducal spray (IBS) was determined by thiobarbituric acid reactive substance assay (TBARS).The influence of pretreatment methods,dilution times of IBS, and the effect of heating were investigated in detail. The results showed that the IBS should be demulsificated by oil phase or 0.01mol L~(-1)HCl solution containing Triton X-100 and 10 times dilution could give better results. It was also found that the increase of LPO in IBS in a year's storage were slight and thus could be negligible. |
| |
| Keywords: | Insulin buccal spray(IBS) Lipid peroxide(LPO) Thiobarbituric acid reactive substances assay(TBARS) |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
