| 灵芝菌丝液体深层发酵种子培养条件研究 |
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| 引用本文： | 吴丽华,仪慧兰,伊晶晶.灵芝菌丝液体深层发酵种子培养条件研究[J].太原师范学院学报(自然科学版),2013(2):129-132. |
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| 作者姓名： | 吴丽华 仪慧兰 伊晶晶 |
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| 作者单位： | [1]太原师范学院生物系,山西太原030031 [2]山西大学生命科学与技术学院,山西太原030006 [3]中国辐射防护研究院放射医学与环境医学研究所,山西太原030006 |
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| 基金项目： | 山西省教育厅应用研发项目 |
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| 摘 要： | 利用液体深层发酵方法进行灵芝菌丝最佳培养基和菌种最适种龄的确定.通过单因素实验和正交实验,研究了不同培养基对液体深层发酵灵芝菌丝生长及多糖含量的影响,确定了灵芝液体深层发酵的培养基配方为:玉米粉3%,豆粉3%,KH2PO40.15%,MgSO40.15%;并且通过研究菌种种龄对液体深层发酵灵芝菌丝生物量及漆酶活性的影响,确定了灵芝液体深层发酵所需菌种的最适种龄为4d.
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| 关 键 词： | 灵芝 培养基 多糖 漆酶 种龄 |
Study on the Culture Conditions for Fermentation Liquid Seed of Ganoderma Lucidum |
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| Wu Lihua Yi Huilan Yi Jingjing.Study on the Culture Conditions for Fermentation Liquid Seed of Ganoderma Lucidum[J].Journal of Taiyuan Normal University:Natural Science Edition,2013(2):129-132. |
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| Authors: | Wu Lihua Yi Huilan Yi Jingjing |
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| Institution: | Wu Lihua Yi Huilan Yi Jingjing (1. Department of Biology,Taiyuan Normal University,Taiyuan 030031; 2. School of Life Science, Shanxi University,Taiyuan 030006 3. Department of Radiological and Environmental, Medicline,China Institute for Radiation Protection,Taiyuan 030006,China) |
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| Abstract: | The optimum culture medium and seed age of Ganoderma Lucidum mycelia were determined by submerged fermentation, and the effects of different optimum culture medium on the growth of Ganoderma Lucidum mycelia and polysaccharide were investigated using single fac- tor and orthogonal experiment in this study. The results showed that the optimum culture medi- um for fermentation liquid seed culture was maize flour 40%, soybean powder 2 %, KH2 PO4 0. 3 %, MgSO40. 075 %. The results also showed that the perfect seed age is 4 days by studying the influ- ence of different seed ages on the growth of Ganoderma Lucidum mycelia and pyrocatechol oxi- dase in the fermentation liquid. |
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| Keywords: | Ganoderma Lucidum medium ingredients polysaccharide laccase seed age |
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