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大白鼠脑组织电极测定鸟嘌呤
引用本文:邓家祺,孔继烈,何红.大白鼠脑组织电极测定鸟嘌呤[J].高等学校化学学报,1992,13(5):583.
作者姓名:邓家祺  孔继烈  何红
作者单位:复旦大学化学系, 上海, 200433
基金项目:国家自然科学基金,长春应用化学研究所电分析化学开放实验室资助
摘    要:以大白鼠脑组织作催化鸟嘌呤脱氨反应的酶源,采用氨气敏电极为基础电极制成测定鸟嘌呤的大白鼠脑组织电极,对其响应特性、介质条件、固定化方法等因素进行了研究,测定鸟嘌呤的E~log鸟嘌呤]曲线斜率为46.5mV,线性范围2.0×10-5~6.3×10-4mol/L,寿命可达28d。

关 键 词:大白鼠脑  鸟嘌呤  组织电极  生物传感器  
收稿时间:1991-04-29

A Sensor for Guanine Using Brain Tissue of Rat
DENG Jia-qi,KONG Ji-lie,HE Hong.A Sensor for Guanine Using Brain Tissue of Rat[J].Chemical Research In Chinese Universities,1992,13(5):583.
Authors:DENG Jia-qi  KONG Ji-lie  HE Hong
Institution:Department of Chemistry, Fudan University, Shanghai, 200433
Abstract:An electrode for the determination of guanine was prepared, which employed brain tissue of rat as the biocatalyst and an ammonia gas sensing electrode as the base electrone.Its response characteristics, buffer conditions and strategies of immobilization were studied.The slope of guanine response curve E-lgGuanine] is 46.5 mV/decade, linear range is 2.0×10-5~6.3×10-4 mol/L, lifetime is over 28 days.
Keywords:Brain tissue of rat  Guanine  Tissue based electrode  Biosensor  
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