| 赤红球菌JDM312环己酮单加氧酶的原核表达及检测 |
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| 引用本文： | 彭哲慧,龚凤娟,刘丹丹,吾鲁木汗·那孜尔别克.赤红球菌JDM312环己酮单加氧酶的原核表达及检测[J].吉首大学学报(自然科学版),2011,32(4):88-91. |
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| 作者姓名： | 彭哲慧 龚凤娟 刘丹丹 吾鲁木汗·那孜尔别克 |
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| 作者单位： | （吉首大学生物资源与环境科学学院，湖南 吉首 416000） |
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| 基金项目： | 湖南省大学生研究性学习和创新性实验计划项目(No.CX2008146) |
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| 摘 要： | 通过酶切法从重组质粒pUC18-chnB中得到编码环己酮单加氧酶的chnB基因序列,将其定向插入原核表达载体pQE30中,构建重组质粒pQE30-chnB,转化到大肠杆菌BL21中并诱导表达目的蛋白,用SDS-PAGE电泳检测表达产物.测序结果表明chnB基因大小为1 623bp,编码由540个氨基酸残基构成的多肽.S...
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| 关 键 词： | 赤红球菌 环己酮单加氧酶 chnB基因 原核表迭 |
Prokaryotic Expression and Detection of Cyclohexanone Monooxygenase from Rhodococcus ruber JDM312 |
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| PENG Zhe-hui,GONG Feng-juan,LIU Dan-dan,NAZIERBIEKE Wulumuhan.Prokaryotic Expression and Detection of Cyclohexanone Monooxygenase from Rhodococcus ruber JDM312[J].Journal of Jishou University(Natural Science Edition),2011,32(4):88-91. |
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| Authors: | PENG Zhe-hui GONG Feng-juan LIU Dan-dan NAZIERBIEKE Wulumuhan |
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| Institution: | (College of Biology and Environmental Sciences,Jishou University,Jishou 41600,China) |
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| Abstract: | The chnB gene encoding a cyclohexanone monooxygenase was isolated from the recombinant plasmid pMD18-chnB by digested with BamHI and HindⅢ,then cloned into the expression vector pQE30 and expressed in E.coli BL21 by IPTG induction.The expressed protein was identified by SDS-PAGE.The sequence analyses showed that the coding region of the chnB of Rhodococcus ruber JDM312 was 1 623 bp in length,and the predicted primary protein was composed of 540 amino acids.The SDS-PAGE analyses revealed a single protein band with a molecular weight of 60 kDa,suggesting that the recombinant plasmid of pQE30-chnB was successfully constructed and the target protein was expressed in E.coli. |
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| Keywords: | rhodococcus ruber cyclohexanone monooxygenase chnB gene prokaryotic expression |
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