Evaluation of extraction methods for the simultaneous analysis of simple and macrocyclic trichothecenes |
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| Authors: | Gü nther Stecher,Kanokwan Jarukamjorn,Rania Bakry,Gü nther K. Bonn |
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| Affiliation: | a Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University of Innsbruck, Innrain 52a, 6020 Innsbruck, Austria
b Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, 40002 Khon Kaen, Thailand |
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| Abstract: | The article is concerned with the simultaneous determination of simple and macrocyclic trichothecenes using high-performance liquid chromatography (LC) coupled to UV and mass spectrometric (MS) detection. Emphasis is put on the liquid-liquid extraction (LLE) and solid phase extraction (SPE) procedure from plant material such as wheat, comparing SPE cartridges packed with different stationary phases based on silica and polymer sorbents. In this coherence a polymeric material on the basis of poly(glycidyl methacrylate-divinylbenzene) (GMA-DVB) is developed with special regard on synthesis procedures to enhance the extraction recovery of trichothecenes in a broad polarity range. Evaluation of extraction techniques showed that the introduced material is competitive with commercially available high quality SPE materials. Percentage recovery is 82% for polar compounds, 89% for medium polar compounds and 98% for lipophilic compounds. |
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| Keywords: | ACN, acetonitrile DON, deoxynivalenol DAS, diacetoxyscirpenol F-X, fusarenone-X H2O, water MeOH, methanol NIV, nivalenol Ro-a, roridin-A T-2, T-2 toxin Ve-a, verrucarin-A VOL, verrucarol GMA-DVB, poly(glycidyl methacrylate-divinylbenzene) |
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