A novel fluorescent method for determination of peroxynitrite using folic acid as a probe |
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| Authors: | Jun-Chao Huang Jun-Chen Diao Jiang-Lan Yuan |
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| Affiliation: | a State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei 430072, China
b Medical College, Zhengzhou University, Zhengzhou, Henan 450052, China |
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| Abstract: | A novel method for the determination of peroxynitrite using folic acid as a fluorescent probe is described. The method is based on the oxidation of the reduced, low-fluorescent folic acid by peroxynitrite to produce a high-fluorescent emission product. The fluorescence increase is linearly related to the concentration of peroxynitrite in the range of 3 × 10−8 to 5.0 × 10−6 mol L−1 with a correlation coefficient of 0.998, and the detection limit is 1 × 10−8 mol L−1. Interferences from some metal ions normally seen in biological samples, and also some anions structurally similar to peroxynitrite were studied. The optimal conditions for the detection of peroxynitrite were evaluated. |
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| Keywords: | Peroxynitrite Folic acid Fluorimetry Probe |
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