Examination of performance of glassy carbon paste electrode modified with gold nanoparticle and xanthine oxidase for xanthine and hypoxanthine detection |
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Authors: | Cubukçu Meliha Timur Suna Anik Ulkü |
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Affiliation: | a Mugla University, Faculty of Arts and Science, Chemistry Department, 48000 Kötekli, Mugla, Turkiye b Ege University, Faculty of Science, Biochemistry Department, 35100 Bornova, Izmir, Turkiye |
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Abstract: | A composite electrode was prepared by modifying glassy carbon microparticles with gold nanoparticles (Au-nps) and xanthine oxidase enzyme (XOD) for xanthine (X) and hypoxanthine (Hx) detection. After the optimization of the system for X, the biosensor was characterized for X and Hx. A linearity was obtained in the concentration range between 5.00 × 10−7 and 1.00 × 10−5 M for X with equation of y = 0.24x + 0.712 and 5.00 × 10−6 to 1.50 × 10−4 M for Hx, with equation of y = 0.014x + 0.575, respectively. Obtained results were compared to X and/or Hx biosensors including/not including Au-np in the structure. The developed system was also applied for detection of Hx in canned tuna fish sample and very promising results were obtained. |
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Keywords: | Gold nanoparticle GCPE Xanthine Hypoxanthine Biosensor |
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