| TOTAL SYNTHESIS OF Trichosanthes TRYPSIN INHIBITOR AND ITS ANALOGUE |
| |
| 引用本文： | 黄种福,吴明磊,戚正武. TOTAL SYNTHESIS OF Trichosanthes TRYPSIN INHIBITOR AND ITS ANALOGUE[J]. 中国科学B辑(英文版), 1990, 0(10) |
| |
| 作者姓名： | 黄种福 吴明磊 戚正武 |
| |
| 作者单位： | Shanghai Institute of Biochemistry，Academia Sinica，Shanghai 200031，PRC，Shanghai Institute of Biochemistry，Academia Sinica，Shanghai 200031，PRC，Shanghai Institute of Biochemistry，Academia Sinica，Shanghai 200031，PRC |
| |
| 基金项目： | This work was supported by the State Biological High Technology Research Grant. |
| |
| 摘 要： | Trichosanthes trypsin inhibitor (TTI) is a peptide consisting of 27 amino acid residues with three pairs of disulfide bonds. This paper reports the total synthesis and disulfide bond refolding of this inhibitor and its analogue. After purification, the amino acid sequence and stoichiometrical inhibitory activity against trypsin of the synthetic inhibitor were compatible with those of the natural inhibitor. The analogue of this inhibitor in which residue Met in position 6 was replaced by Ala was also synthesized. The antitrypsin activity of this synthetic analogue was also approximate to that of the natural inhibitor.
|
TOTAL SYNTHESIS OF Trichosanthes TRYPSIN INHIBITOR AND ITS ANALOGUE |
| |
| HUANG ZHONG-FU WU MING-LEI AND QI ZHENG-WU. TOTAL SYNTHESIS OF Trichosanthes TRYPSIN INHIBITOR AND ITS ANALOGUE[J]. Science in China(Chemistry), 1990, 0(10) |
| |
| Authors: | HUANG ZHONG-FU WU MING-LEI AND QI ZHENG-WU |
| |
| Abstract: | Trichosanthes trypsin inhibitor (TTI) is a peptide consisting of 27 amino acid residues with three pairs of disulfide bonds. This paper reports the total synthesis and disulfide bond refolding of this inhibitor and its analogue. After purification, the amino acid sequence and stoichiometrical inhibitory activity against trypsin of the synthetic inhibitor were compatible with those of the natural inhibitor. The analogue of this inhibitor in which residue Met in position 6 was replaced by Ala was also synthesized. The antitrypsin activity of this synthetic analogue was also approximate to that of the natural inhibitor. |
| |
| Keywords: | peptide synthesis squash family inh(?)bitors Trichosanthes trypsin inhibitor disulfide bond refolding |
| 本文献已被 CNKI 等数据库收录！ |
