Analytical fractionation of aquatic humic substances by metal affinity chromatography on iron(III)-coated cellulose |
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Authors: | R Kuckuk and P Burba |
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Institution: | (1) Institute of Spectrochemistry and Applied Spectroscopy (ISAS), D-44139 Dortmund, Germany, DE |
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Abstract: | The chromatographic fractionation of aquatic humic substances (HS) onto iron(III)-coated cellulose (Cell-Fe(III)) as a metal-loaded
adsorbent is described, analogously to the separation principle of the well-established metal affinity chromatography (MAC).
For that purpose the sorption of HS from different aquatic origin on that collector was characterized by their kinetics and
equilibrium distribution coefficients Kd. Based on Kd values of 103 to 104,mL/g, and fast sorption kinetics a preparative HPLC procedure, using stepwise increased pH-values (pH 8–12.5, borate buffer)
as an eluent, was developed for the fractionation of dissolved HS (up to 7 fractions of different amount). The fractions obtained
by this MAC procedure from selected aquatic HS samples were different in their Cu(II) complexation capacity, absorbance ratio
E265 nm/E365 nm and Fourier transform infrared spectra.
Received: 14 June 1999 / Revised: 6 August 1999 / Accepted: 10 August 1999 |
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