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| 腐胺对苜蓿组织培养中球形胚发生的作用研究 | |
| 引用本文： | 刘天磊,王一欣,王仑山.腐胺对苜蓿组织培养中球形胚发生的作用研究[J].兰州大学学报(自然科学版),2003,39(4):62-65. |
| 作者姓名： | 刘天磊 王一欣 王仑山 |
| 作者单位： | 1. 兰州大学,生命科学学院,甘肃,兰州,730000;复旦大学生命科学学院遗传研究所,上海,200433 2. 深圳科粉生物制品有限公司,广东,深圳,518057 3. 兰州大学,生命科学学院,甘肃,兰州,730000 |
| 摘 要： | 苜蓿下胚轴外植体诱导的胚性愈伤组织转至分化培养基上形成球形胚，利用这一实验体系研究了腐胺及多胺代谢抑制剂MBGB、CHA对球形胚发生频率的影响，同时对DNA、RNA和可溶性蛋白含量变化的测定表明，其与球形胚的发生具有相关性. |
| 关 键 词： | 苜蓿 球形胚 腐胺 甲基乙二醛-双脒基腙 环己胺 |
| 文章编号： | 0455-2059(2003)04-0062-04 |
| 修稿时间： | 2002年10月9日 |
Studies on functions of putrescine in globular embryogenesis of tissue culture of Medicago sativa L. |
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| LIU Tian lei ,WANG Yi xin ,WANG Lun shan.Studies on functions of putrescine in globular embryogenesis of tissue culture of Medicago sativa L.[J].Journal of Lanzhou University(Natural Science),2003,39(4):62-65. | |
| Authors: | LIU Tian lei WANG Yi xin WANG Lun shan |
| Institution: | LIU Tian lei 1,2,WANG Yi xin 3,WANG Lun shan 1 |
| Abstract: | Callus of Medicago sativa L.from the explants of hypocotyledons was stepping into the period of globular embryogenesis while being cultivated on a medium for inducing differentiation.With excellent experimental system,the effect of putrescine,MGBG and CHA of polyamine metabolic inhibitors on the frequency of globular embryogenesis was probed,while bio macromolecular metabolism was also monitored and the data indicated that those in erratic changes were related to globular embryogenesis. |
| Keywords: | Medicago sativa L globular embryo putrescine MGBG CHA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |