KINETIC BEHAVIOR OF EPR SIGNAL I: INDUCTION EFFECTS IN INTACT ALGAE |
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Authors: | Ellen C. Weaver |
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Affiliation: | Biophysics Laboratory, W. W. Hansen Laboratories of Physics, Stanford University |
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Abstract: | Abstract— There is considerable evidence that the light-induced narrow EPR signal I at g = 2·0025 in green algae is a reflection of the turnover of the photosynthetic reaction center, P700. A lag period or induction effect of the formation of this signal, subsequent to a dark period in whole fresh cells, is similar to induction effects for the bleaching of P700. The rate of formation of the signal is slower the longer the preceding dark period. DCMU does not much affect the induction phenomenon, but heating the cells to 60° abolishes it. Some substance or process which exists in the intact cell is altered by light and is detectable by its effect on signal I and on the rate of light-induced oxidation of P700 and cytochrome. |
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