高效液相色谱法测定糖脂双降茶中淫羊藿甙的含量

 建立了高效液相色谱法测定糖脂双降茶中淫羊藿甙含量的方法。采用ODS柱 ,以甲醇 水 (体积比为 60∶4 0 )为流动相 ,用紫外检测器于 2 70nm处检测。结果表明 ,淫羊藿甙的质量浓度在 0 1g/L～ 0 5 g/L范围内与色谱峰的峰面积呈良好的线性关系 (r =0 9993 ) ,回收率为 10 1 0 %～ 10 1 7% ,RSD为 1 6%～ 2 7% (n =3 )。方法快速、简便、准确 ,结果稳定 ,重现性好 ,可推荐其作为质量检验的一个定量方法。

Quantitative Determination of Icariin in "Tangzhi Shuangjiang Cha" by High Performance Liquid Chromatography

Icariin in "Tangzhi Shuangjiang Cha" was quantitatively determined by HPLC with ODS column, a mixture of MeOH-H2O(60:40, V/V) as mobile phase and UV detection at 270 nm. There was a good linear relationship within the range of 0.1 g/L-0.5 g/L(r = 0.9993). The average recovery was 101.4%, RSD was below 2.7%(n = 3). The method is rapid, simple, accurate and reproducible. And the method is proposed to be used for quality control of "Tangzhi Shuangjiang Cha".