| 中性蛋白酶修饰条件的优化 |
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| 引用本文： | 方林明,李德才,赵世光. 中性蛋白酶修饰条件的优化[J]. 安徽工程科技学院学报：自然科学版, 2014, 0(2): 13-16 |
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| 作者姓名： | 方林明 李德才 赵世光 |
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| 作者单位： | 安徽工程大学生物与化学工程学院,安徽芜湖241000 |
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| 基金项目： | 安徽省自然科学基金资助项目（11040606M81） |
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| 摘 要： | 以氰脲酰氯活化的单甲氧基聚乙二醇5000(mPEG 5000)对中性蛋白酶进行化学修饰,并研究其修饰条件对修饰率的影响.正交试验结果表明,mPEG 5000修饰中性蛋白酶的最佳条件为pH值7、修饰温度45℃、酶质量浓度1.8g/L、反应时间5.5h,此条件下修饰率可达到51.9%,为下一步研究修饰中性蛋白酶的酶学性质提供了理论依据.
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| 关 键 词： | 单甲氧基聚乙二醇 中性蛋白酶 化学修饰 正交试验 |
Optimization of modified conditions for neutral protease |
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| FANG Lin-ming,LI De-cai,ZHAO Shi-guang. Optimization of modified conditions for neutral protease[J]. Journal of Anhui University of Technology and Science, 2014, 0(2): 13-16 |
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| Authors: | FANG Lin-ming LI De-cai ZHAO Shi-guang |
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| Affiliation: | (Coll. of Bioch. Engn. , Anhui Polytechnic University,Wuhu 241000,China) |
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| Abstract: | The monomethoxypolyethylene glycol 5000 （mPEG 5000）, activated with cyanuric chloride, chemically modified the neutral protease, then the influence of the modification rate of the modified con- ditions and optimization was studied. The result of orthogonal test showed that mPEG 5000 optimal conditions for modification of the neutral protease pH was 7, modified temperature was 45℃ ,enzyme concentration was 1.8 g/L,the reaction time was 5.5 h. Under this condition the modification rate can reach 51.9 ~ , which provides the theoretical basis for further study on the modified neutral protease enzymatic properties. |
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| Keywords: | neutral protease chemical modification monomethoxypolyethylene glycol （mPEG） orthogonal test |
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