Assay for urinary estriol during the menstrual cycle based on extraction by a graphitized carbon black cartridge followed by high-performance liquid chromatography |
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Authors: | Guia Carfagnini A. Di Corcia M. Marchetti R. Samperi |
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Affiliation: | (1) Dipartimento di Chimica, Università La Sapienza di Roma, Piazza Aldo Moro, 00185 Roma, Italy |
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Abstract: | Summary A high-performance liquid chromatography (HPLC) assay for estriol in nonpregnancy urine is described. After Enzymic hydrolysis, the estriol is extracted from urine by the sorbent trap technique utilizing graphitized carbon black (Carbopack B). After some washing steps, estriol is desorbed by a suitable solvent system. After solvent removal, the sample is injected into an HPLC column for estriol quantification. Analytical recovery of estriol was 96.1%. The precision of the method was 2.6 and 4.9% respectively at 145 and 10.6ng/ml of urine. The limit of sensitivity was set at 0.8 ng/ml of urine. The mean contents of estriol in the follicular and luteal phases were respectively 11.3 and 38.8 ng/ml of urine.Dedicated to Prof. Dr. A. Liberti on the occasion of his 70th birthday. |
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Keywords: | Estriol Urine Menstrual Cycle Column liquid chromatography |
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