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Far‐Red Organic Fluorophores Contain a Fluorescent Impurity
Authors:Matthew B. Stone  Prof. Sarah L. Veatch
Affiliation:Department of Biophysics, University of Michigan, 930 N University, Ann Arbor MI 48109 (USA)
Abstract:Far‐red organic fluorophores commonly used in traditional and super‐resolution localization microscopy are found to contain a fluorescent impurity with green excitation and near‐red emission. This near‐red fluorescent impurity can interfere with some multicolor stochastic optical reconstruction microscopy/photoactivated localization microscopy measurements in live cells and produce subtle artifacts in chemically fixed cells. We additionally describe alternatives to avoid artifacts in super‐resolution localization microscopy.
Keywords:fluorescence  fluorescent probes  live cells  quantitative imaging  super‐resolution imaging
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