Far‐Red Organic Fluorophores Contain a Fluorescent Impurity |
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| Authors: | Matthew B Stone Prof Sarah L Veatch |
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| Institution: | Department of Biophysics, University of Michigan, 930 N University, Ann Arbor MI 48109 (USA) |
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| Abstract: | Far‐red organic fluorophores commonly used in traditional and super‐resolution localization microscopy are found to contain a fluorescent impurity with green excitation and near‐red emission. This near‐red fluorescent impurity can interfere with some multicolor stochastic optical reconstruction microscopy/photoactivated localization microscopy measurements in live cells and produce subtle artifacts in chemically fixed cells. We additionally describe alternatives to avoid artifacts in super‐resolution localization microscopy. |
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| Keywords: | fluorescence fluorescent probes live cells quantitative imaging super‐resolution imaging |
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