Imaging sclera with hard X-ray microscopy |
| |
Authors: | Fullwood Nigel J Martin Francis L Bentley Adam J Lee Jin Pyung Lee Sang Joon |
| |
Affiliation: | Division of Biomedicine and Life Sciences, School of Heath and Medicine, Lancaster University, Lancaster, UK. n.fullwood@lancaster.ac.uk |
| |
Abstract: | In this study, the organization of collagen fibrils within the sclera of the eye was investigated using the 7 keV hard X-ray microscope of the Pohang light source and compared to images from electron and atomic force microscopy. From the captured X-ray images, individual collagen fibrils were observed clearly in a spatial resolution much better than 100 nm, both in longitudinal sections and in transverse sections. Some of the collagen fibrils showed evidence of axial periodicity. In some regions of the samples, we could see cross-bridge like structures between adjacent collagen fibrils. The X-ray microscope also allowed the observation of keratocytes and the lamella structure of the scleral stroma. The X-ray microscope has some unique advantages in the nano-scale imaging of bio-samples relative to other established imaging techniques. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|