Direct enantiomeric separation of saterinone by high-performance liquid chromatography

A new method is described for the direct liquid chromatographic separation and determination of the enantiomers of saterinone, 1-[(4-cyano-1,2-dihydro-6-methyl-2-oxopyridinyl-5)-phenoxy]-3-[4-( 2- methoxyphenyl)piperazinyl-1]-propan-2-ol, using a Chiralcel OD column with methanol as eluent and a temperature set to 10 degrees C. Several lots of synthesized enantiomers were analysed for their enantiomeric purity of more than 99%, because of the excellent resolution (Rs = 2.2). Furthermore the enantiomeric ratio was determined in plasma samples after oral and intravenous administration of racemic saterinone.