Novel Fluorophores as Building Blocks for Optical Probes for In Vivo Near Infrared Fluorescence (NIRF) Imaging |
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Authors: | Jutta Pauli Robert Brehm Monika Spieles Werner A Kaiser Ingrid Hilger and Ute Resch-Genger |
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Institution: | 1.BAM Federal Institute for Materials Research and Testing, Division I.5,Berlin,Germany;2.Institut für Diagnostische und Interventionelle Radiologie des Klinikums der Friedrich-Schiller-Universit?t Jena (IDIR), Forschungszentrum Lobeda,Jena,Germany |
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Abstract: | Aiming at the identification of new fluorescent reporters for targeted optical probes, we assessed the application-relevant
features of a novel asymmetric cyanine, DY-681, in comparison to the only clinically approved dye indocyanine green (ICG),
the golden imaging standard Cy5.5, and the asymmetric cyanine DY-676 successfully exploited by us for the design of different
contrast agents. This comparison included the analysis of the spectroscopic properties of the free fluorophores and their
thermal stability in aqueous solution as well as their cytotoxic potential. In addition, the absorption and emission features
of IgG-conjugated DY-681 were examined. The trimethine DY-681 exhibited spectral features closely resembling that of the pentamethine
Cy5.5. Its high thermal stability in phosphate buffer saline (PBS) solution in conjunction with its low cytotoxicity, reaching
similar values as determined for Cy5.5 and DY-676, renders this dye more attractive as ICG and, due to its improved fluorescence
quantum yield in PBS, also superior to DY-676. Although in PBS, Cy5.5 was still more fluorescent, the fluorescence quantum
yields (Φ
f) of DY-681 and Cy5.5 in PBS containing 5 mass-% bovine serum albumin (BSA) were comparable. Labeling experiments with DY-681
and the model antibody IgG revealed promisingly high Φ
f values of the bioconjugated dye. |
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