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A kit of human polyclonal IgG for the diagnosis of infectious processes |
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| Authors: | A Perera C Perez M B Torres A Henandez F C Heres T Moreira A Gutierrez A Gigato I Hernandez L Alberti O Marrero L Martinez J M Sanfiz E Sanchez J Rodriguez L O Marrero G Parra |
| Institution: | (1) Center for Clinical Research, Havana, Cuba;(2) Institute for Cardiology and Cardiovascular Surgery, Havana, Cuba;(3) National Center for Scientific Researches, Havana, Cuba;(4) Isotopes Center, Havana, Cuba;(5) National Institute for Oncology and Radiobiology, Havana, Cuba;(6) Orthopedic Hospital “Frank Pais”, Havana, Cuba |
| Abstract: | The aim of this work was to obtain a freeze-dried kit for direct99mTc-labeling of human polyclonal IgG. The labeling procedure was carried out by Schwarz's method. The best yields of99mTc-IgG were obtained by using sodium pyrophosphate decahydrate as a weak chelating agent. Performed tests showed the stability of the radiopharmaceutical up to 24 hours. Plasma clearance in rats was fitted to a biexponential curve withT 1/2α=(0.1 ±0.9) h andT 1/2β=(10±3) h. The organs with higher uptake of radiopharmaceutical were lung, kidneys and blood. In a rabbit model the abscess target/background ratio was 3–6 according to time of the scintigraphic images. Thirty patients with musculoskeletal infection were studied. Twenty-one lesions were detected and confirmed by culture/biopsy. |
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