Oligonucleotide nanostructured surfaces: effect on Escherichia coli curli expression |
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Authors: | Cottenye Nicolas Teixeira Francisco Ponche Arnaud Reiter Günter Anselme Karine Meier Wolfgang Ploux Lydie Vebert-Nardin Corinne |
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Institution: | Institut de Chimie des Surfaces et Interfaces, CNRS UPR 9069, Mulhouse Cedex, France. |
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Abstract: | Oligonucleotide model surfaces allowing independent variation of topography and chemical composition were designed to study the adhesion and biofilm growth of E.coli. Surfaces were produced by covalent binding of oligonucleotides and immobilization of nucleotide-based vesicles. Their properties were confirmed through a combination of fluorescence microscopy, XPS, ellipsometry, AFM and wettability studies at each step of the process. These surfaces were then used to study the response of three different strains of E.coli quantified in a static biofilm growth mode. This study led to convincing evidence that oligonucleotide-modified surfaces, independent of the topographical feature used in this study, enhanced curli expression without an increase in the number of adherent bacteria. |
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Keywords: | biological applications of polymers block copolymers self‐assembly surfaces vesicles |
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