Orientation-selective incorporation of transmembrane F0F1 ATP synthase complex from micrococcus luteus in polymer-supported membranes |
| |
Authors: | Tutus Murat Rossetti Fernanda F Schneck Emanuel Fragneto Giovanna Förster Friedrich Richter Ralf Nawroth Thomas Tanaka Motomu |
| |
Affiliation: | Biophysical Chemistry Laboratory and Center for Quantitative Biology (BIOQUANT), University of Heidelberg, 69120 Heidelberg, Germany. |
| |
Abstract: | We report the vectorial incorporation of a highly asymmetric F0F1 ATP synthase complex from Micrococcus luteus into polymer-supported membranes. Dynamic light scattering and cryo electron microscopy confirm that the use of weak surfactants (bile acid) allows for the non-disruptive protein incorporation into lipid vesicles. Spreading of vesicles with ATP synthase onto a cellulose support results in a homogeneous distribution of proteins, in contrast to a patchy image observed on bare glass slides. The orientation of ATP synthase can be identified using an antibody to the ATP binding site as well as from topographic profiles of the surface. The method to "align" transmembrane proteins in supported membranes would open a possibility to quantify protein functions in biomimetic model systems. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |