Application of preparative high-speed counter-current chromatography for separation of methyl gallate from Acer truncatum Bunge |
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| Authors: | Ma Xiaofeng Wu Linhuan Ito Yoichiro Tian Weixi |
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| Affiliation: | Department of Biology, The Graduate School of Chinese Academy of Sciences, Beijing 100049, China. |
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| Abstract: | Preparative separation of methyl gallate in leaves extract of Acer truncatum Bunge was conducted using high-speed counter-current chromatography (HSCCC) with a solvent system composed of ethyl acetate-ethanol-water at volume ratios of 5:1:5 (v/v/v). In a single operation, 57.5 mg of methyl gallate was obtained from 120 mg of the extract. HPLC analyses of the counter-current chromatography (CCC) fraction revealed that the methyl gallate was having over 97% purity. Its structure was identified by 1H NMR and 13C NMR. |
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