连串反应停流速差动力学分析同时测定肾上腺素和去甲肾上腺素

首次将连串反应用于速差动力学分析, 测定了具有广泛生物活性的肾上腺素和去甲肾上腺素的含量。对于连串反应A^k^1→B^k^2→C, 由于动力学方程与两个速率常数k1和k2有关, 混合体系的k1或者k2有差异, 都能用于速差动力学分析, 这与利用简单反应进行速差动力学分析有所不同, 为提高速差提供了更多的可能性。三-(1,10-邻菲罗林)-铁(III)配合物氧化肾上腺素或去甲肾上腺素的过程为一连串反应, 两者的k1相近而k2差异显著, 利用k2的差异可以进行两者的同时测定。对于10^-^5mol.dm^-^3级的肾上腺素-去甲肾上腺素混合物, 比例范围在1:6.88到2.91:1之间, 测定误差小于±5%, 平均精密度为2.5%。

Simultaneous determination of epinephrine and norepinephrine by stopped-flow differential kinetic analysis based on successive reactions

For the first time, successive reactions was used in differential kinetic analysis, and simultaneous determination of epinephrine and norepinephrine based on it was carried out. For successive reaction A^k^1→B^k^2→C, because the integral kinetic equation have relationship with two rate constants K1 and K2, two rate constants can be used in differential kinetic analysis, this afford more possibility to increase the kinetic difference between mixed species. The successive reaction between tris (1,10-phenanthroline)-iron(III) and epinephrine or norepinephrine was investigated, it was found that between the two species, k1 was similar, but k2 had enough difference, this was exploited by conventional proportional-equation method to resolution of epinrphrine-norepine-phrine mixture at the 10^-^5 mol.dm^-^3 level over the ratio range 1:6.88-2.91:1, with an error of less than ±5% and an average precision of about 2.5%.