Solid-phase extraction—Thermal desorption—Gas chromatography with mass selective detection for the determination of drugs in urine |
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Authors: | M W J van Hout R A de Zeeuw J P Franke G J de Jong |
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Institution: | (1) Department of Analytical Chemistry and Toxicology, University Centre for Pharmacy, University of Groningen, A. Deusinglaan 1, 9713 AV Groningen, The Netherlands;(2) Present address: Department of Biomedical Analysis, Faculty of Pharmacy, University of Utrecht, P.O Box 80082, 3508 TB Utrecht, The Netherlands |
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Abstract: | Summary Solid-phase extraction (SPE) was combined with thermal desorption (TD) and gas chromatographic (GC) analysis to determine
drugs in urine. The extrattion was performed inside a fritted GC liner using about 5 mg TENAX that was inserted into the liner
on top of the frit. After extraction, the liner was placed into the injector of the GC and the analytes were thermally desorbed
by using a programmed-temperature vaporiser. Several sorbent materials were investigated for the applicability of SPETD-GC
analysis. TENAX proved to be the most suitable sorbent, since hardly any interferences were observed and acceptable absolute
recoveries (73 and 74%) were obtained for lidocaine and diazepam. A mass selective detector (MSD) in the selected ion monitoring
mode allowed detection of lidocaine and diazepam down to 0.5 ng·mL−1 using 50μL urine. The use of only 5 mg of extraction material allowed rapid extraction, while a 10 m GC column provided a
fast chromatographic system. As a results, the total analysis time was less than 20 min, including 5 min for drying the TENAX
and 5 min for thermal desorption. Thus, SPETD-GC-MS appears to be a powerful tool for the rapid analysis of biological samples. |
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Keywords: | Gas chromatography— mass spectrometry Solid-phase extraction-thermal desorption Programmed-temperature vaporiser Lidocaine and diazepam in urine |
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