Purification of astilbin and isoastilbin in the extract of smilax glabra rhizome by high-speed counter-current chromatography |
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Authors: | Du Qizhen Li Lei Jerz Gerold |
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Institution: | Institute of Food and Biological Engineering, Zhemjiang Gongshang University, 149 Jiaogong Road, Hangzhou, Zhejiang 310035, China. qizhendu@mail.zjgsu.edu.cn |
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Abstract: | Purification of astilbin from the rhizome extract of Smilax glabra was conducted using a high-speed counter-current chromatograph equipped with a 700 mL column. In a single operation, 1.5 g of crude sample was separated to yield 105 mg of component astilbin and 48 mg of isoastilbin while the upper phase of the two-phase solvent system composed of n-hexane-n-butanol-water (1:1:2, v/v/v) was used for stationary phase. The chemical structures of the two flavonoid glycosides were confirmed by electrospray ionization ion trap multiple mass spectrometry and nuclear magnetic resonance experiments. |
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Keywords: | Counter-current chromatography Liliaceae Astilbin Purification |
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