| 血清胸腺因子(FTS)的合成和活性测定 |
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| 引用本文： | 廖晓全,周立娜,赵宗阁,徐康森,袁勤生.血清胸腺因子(FTS)的合成和活性测定[J].华东理工大学学报(自然科学版),2005,31(6):727-730. |
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| 作者姓名： | 廖晓全 周立娜 赵宗阁 徐康森 袁勤生 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室,上海,200237;中国医科大学基础医学院,沈阳,110001;中国药品生物制品检定所,北京,100050 |
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| 摘 要： | 采用芴甲氧羰基（Fmoc）固相肽合成法（Fmoc～SPPS）合成了血清胸腺因子（FTS），用中压液相阴离子柱一步纯化，经HPLC分析纯度，通过质谱和氨基酸序列测定鉴定合成产物。根据FTS恢复胸腺切除小鼠的脾细胞对免疫抑制荆AZ敏感的特性测定合成FTS的活性。结果表明：化学合成血清胸腺因子的产率为94．7％；纯化后其纯度达到93．7％；质谱测定其相对分子质量为876．6，与理论分子量相符。氨基酸序列测定为NH2／Glu—Ala—Lys—Ser—Gin—Gly—Gly—Ser—Asn，与设计序列一致。实验显示在合成FTS的浓度为10^-14mol／L时尚有部分活力，在10^-13mol／L时能够完全恢复胸腺切除小鼠的脾细胞对AZ的敏感性。
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| 关 键 词： | 血清胸腺因子 合成 胸腺切除 |
| 文章编号： | 1006-3080(2005)06-0727-04 |
| 收稿时间： | 2004-11-27 |
| 修稿时间： | 2004年11月27 |
Synthesis and Activity Determination of Thymulin(FTS) |
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| LIAO Xiao-quan,ZHOU Li-na,ZHAO Zong-ge,XU Kang-sen,YUAN Qin-sheng.Synthesis and Activity Determination of Thymulin(FTS)[J].Journal of East China University of Science and Technology,2005,31(6):727-730. |
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| Authors: | LIAO Xiao-quan ZHOU Li-na ZHAO Zong-ge XU Kang-sen YUAN Qin-sheng |
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| Institution: | 1. Stake Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; 2. College of Basic Medical Science, China Medical University, Shenyang 110001, China; 3. National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100056, China |
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| Abstract: | Thymulin(FTS) was synthesized by Fmoc-SPPS,purified by MPLC,and identified by HPLC,MS and amino acid sequence determination.Biological activity of FTS was measured according to its characteristic of conferring on spleen cells from adult thymectomized that mice sensitivity to azathioprine(AZ) identical to that of normal mouse spleen cells.Results show the purity of the product was (93.7%);the molecular mass was 876.6;the amino acid sequence was NH_2/Glu-Ala-Lys-SerGln-Gly-Gly-Ser-Asn.The synthesized FTS can completely restore spleen cells from thymectomized mice sensitivity to AZ in the concentration of 10~(-13) mol/L. |
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| Keywords: | thymulin synthesis thymectomy |
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