Online photolytic optical gating of caged fluorophores in capillary zone electrophoresis utilizing an ultraviolet light‐emitting diode |
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| Authors: | Elyssia S Gallagher Troy J Comi Kevin L Braun Craig A Aspinwall |
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| Institution: | 1. Department of Chemistry and Biochemistry, University of Arizona, , Tucson, AZ, USA;2. Department of Chemistry, Beloit College, , Beloit, WI, USA;3. Bio5 Institute, University of Arizona, , Tucson, AZ, USA |
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| Abstract: | Photolytic optical gating (POG) facilitates rapid, on‐line and highly sensitive analyses, though POG utilizes UV lasers for sample injection. We present a low‐cost, more portable alternative, employing an ultraviolet light‐emitting diode (UV‐LED) array to inject caged fluorescent dyes via photolysis. Utilizing the UV‐LED array, labeled amino acids were injected with nanomolar limits of detection (270 ± 30 nM and 250 ± 30 nM for arginine and citrulline, respectively). When normalized for the difference in light intensity, the UV‐LED array provides comparable sensitivity to POG utilizing UV lasers. Additionally, the UV‐LED array yielded sufficient beam quality and stability to facilitate coupling with a Hadamard transform, resulting in increased sensitivity. This work shows, for the first time, the use of an UV‐LED for online POG with comparable sensitivity to conventional laser sources but at a lower cost. |
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| Keywords: | Capillary zone electrophoresis Hadamard transform Photolytic optical gating Ultraviolet light‐emitting diode |
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