Retrotransposon‐microsatellite amplified polymorphism,an electrophoretic approach for studying genetic variability among Schistosoma japonicum geographical isolates |
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| Authors: | Juan Li Guang‐Hui Zhao Dong‐Hui Zhou Hiromu Sugiyama Alasdair J. Nisbet Xiao‐Yan Li Feng‐Cai Zou Hai‐Long Li Lin Ai Xing‐Quan Zhu |
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| Affiliation: | 1. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, , Lanzhou, Gansu Province, P. R. China;2. South China Agricultural University, , Guangdong Province, P. R. China;3. Institute of Veterinary Medicine, Guangdong Academy of Agricultural Sciences, , Guangzhou, Guangdong Province, P. R. China;4. Northwest A & F University, , Yangling, Shaanxi Province, P. R. China;5. Department of Parasitology, National Institute of Infectious Diseases, , Tokyo, Japan;6. Parasitology Division, Moredun Research Institute, , Midlothian, Scotland, UK;7. College of Animal Science and Technology, Yunnan Agricultural University, , Yunnan Province, P. R. China;8. School of Basic Medicine, Dali University, , Dali, Yunnan Province, P. R. China;9. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, , Shanghai, P. R. China |
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| Abstract: | In the present study, retrotransposon‐microsatellite amplified polymorphism (REMAP) was used to examine genetic variability among Schistosoma japonicum isolates from different endemic provinces in mainland China, using S. japonicum from Japan and the Philippines for comparison. Of the 50 primer combinations screened, eight produced highly reproducible REMAP fragments. Using these primers, 190 distinct DNA fragments were generated in total, of which 147 (77.37%) were polymorphic, indicating considerable genetic variation among the 43 S. japonicum isolates examined. The percentage of polymorphic bands (PPB) among S. japonicum isolates from mainland China, Japan, and the Philippines was 77.37%; PPB values of 18.42% and 53.68% were found among isolates from southwestern (SW) China and the lower Yangtze/Zhejiang province in eastern (E) China, respectively. Based on REMAP profiles, unweighted pair‐group method with arithmetic averages (UPGMA) dendrogram analysis revealed that all of the S. japonicum samples grouped into three distinct clusters: parasites from mainland China, Japan, and the Philippines were clustered in each individual clade. Within the mainland China cluster, SW China isolates (from Sichuan and Yunnan provinces) grouped together, whereas worms from E China (Zhejiang, Anhui, Jiangxi, Jiangsu, Hunan, and Hubei provinces) grouped together. These results demonstrated that the REMAP marker system provides a reliable electrophoretic technique for studying genetic diversity and population structures of S. japonicum isolates from mainland China, and could be applied to other pathogens of human and animal health significance. |
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| Keywords: | China Genetic diversity Retrotransposon‐microsatellite amplified polymorphism (REMAP) Retrotransposons Schistosoma japonicum |
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