Verification and Biophysical Characterization of a New Three‐Color Förster Resonance‐Energy‐Transfer (FRET) System in DNA |
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| Authors: | Andrea Altevogt?née?Kienzler Roman Flehr Sören Gehne Michael U Kumke Willi Bannwarth |
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| Institution: | 1. Albert‐Ludwigs University of Freiburg, Institute of Organic Chemistry and Biochemistry, Albertstr. 21, D‐79104 Freiburg (phone: +49‐761‐2036073;2. fax: +49‐761‐2038705);3. University of Potsdam, Institute of Chemistry (Physical Chemistry), Karl‐Liebknecht‐Str. 24?–?25, D‐14476 Potsdam‐Golm |
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| Abstract: | We report on a new three‐color FRET system consisting of three fluorescent dyes, i.e., of a carbostyril (=quinolin‐2(1H)‐one)‐derived donor D, a (bathophenanthroline)ruthenium complex as a relay chromophore A1, and a Cy dye as A2 (FRET=Förster resonance‐energy‐transfer) (cf. Fig. 1). With their widely matching spectroscopic properties (cf. Fig. 2), the combination of these dyes yielded excellent FRET efficiencies. Furthermore, fluorescence lifetime measurements revealed that the long fluorescence lifetime of the Ru complex was transferred to the Cy dye offering the possibility to measure the whole system in a time‐resolved mode. The FRET system was established on double‐stranded DNA (cf. Fig. 3) but it should also be generally applicable to other biomolecules. |
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| Keywords: | Fö rster resonance energy transfer (FRET) system DNA Fluorescence Ruthenium complexes |
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