Amperometric Determination of Creatinine with a Dialysis Membrane‐Covered Nitrobenzene/Water Interface for Urine Analysis |
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| Authors: | Risa Matsui Toru Sakaki Toshiyuki Osakai |
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| Institution: | 1. Department of Chemistry, Graduate School of Science, Kobe University, Nada, Kobe 657‐8501, Japan;2. A&T Corporation, 2023‐1 Endo, Fujisawa, Kanagawa 252‐0816, Japan |
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| Abstract: | The ion transfer of creatinine (CRE) at a polarized nitrobenzene (NB)/water (W) interface has been studied. When the pH of the W phase is in the range of 1.2 to 4.0, a well‐defined voltammetric wave is observed for a simple transfer of CRE+ (protonated form) at the NB/W interface. This transfer reaction has been applied to develop an amperometric method for the determination of CRE in urine. In this system, the NB/W interface is covered with a dialysis membrane to prevent possible interference from urine proteins. The concentration of CRE in a urine control has successfully been determined. |
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| Keywords: | Creatinine Amperometry ITIES Dialysis membrane Urine analysis |
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