Flow Potentiometric Injection Analysis of Uric Acid Using Lipid Stabilized Films with Incorporated Uricase on ZnO Nanowires |
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| Authors: | Nikolaos Tzamtzis Vasillios N Psychoyios Georgia‐Paraskevi Nikoleli Dimitrios P Nikolelis Nikolas Psaroudakis Magnus Willander Muhammad Qadir Israr |
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| Institution: | 1. Laboratory of Inorganic & Analytical Chemistry, School of Chemical Engineering, Dept 1, Chemical Sciences, National Technical University of Athens, 9 Iroon Polytechniou St., Athens 157 80, Greece;2. Laboratory of Environmental Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis‐Kouponia, GR‐15771 Athens, Greece tel: ++302107274754;3. Laboratory of Inorganic Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis‐Kouponia, GR‐15771 Athens, Greece;4. Department of Science and Technology, Campus Norrk?ping, Link?ping University, SE‐60174 Norrk?ping, Sweden |
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| Abstract: | A novel potentiometric uric acid biosensor was fabricated by immobilization of uricase into stabilized lipid films using zinc oxide (ZnO) nanowires as measuring electrode. Uricase was incorporated into the lipid film prior polymerization on the surface of well aligned ZnO nanowires resulting in a sensitive, selective, stable and reproducible uric acid biosensor. The potentiometric response was twice as large from previously reported values due to the presence of a cationic lipid in the lipid film. The sensor response had no interferences by normal concentrations of ascorbic acid, glucose, urea, proteins and lipids. |
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| Keywords: | ZnO Nanowires Potentiometric nanosensors Uricase Uric acid Stabilized lipid films Implanted electrochemical nanodevices |
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