A Nitrite Biosensor Based on the Direct Electron Transfer of Hemoglobin Immobilized on Carboxyl‐Functionalized Multiwalled Carbon Nanotubes/Polyimide Composite |
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| Authors: | Huanhuan Kou Lingpu Jia Chunming Wang Weichun Ye |
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| Affiliation: | 1. State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, P.?R. China tel.: +?86?09318911895;2. fax: +?86?09318912582;3. College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P.?R. China |
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| Abstract: | The high electrically conductive carboxyl‐functionalized multiwalled carbon nanotubes (COOH‐MWCNTs) were used to combine with nonconducting polyimide (PI) to generate a PI/COOH‐MWCNTs membrane. PI served as a matrix to entrap COOH‐MWCNTs and hemoglobin (Hb). COOH‐MWCNTs can improve the conductivity of the composite. The direct electrochemistry measurement indicated that the PI/COOH‐MWCNTs composite enhanced the immobilization of Hb significantly. Besides, the Hb/PI/COOH‐MWCNTs/GCE biosensor possessed excellent electrocatalytic activity for the detection of nitrite. Therefore, PI is a good matrix for Hb immobilization and it has application in sensor construction. This work is promising in the development of sensitive biosensors based on PI/COOH‐MWCNTs composite film. |
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| Keywords: | Biosensors Hemoglobin Multiwalled carbon nanotubes Polyimide |
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