Study of Inhibition,Reactivation and Aging Processes of Pesticides Using Graphene Nanosheets/Gold Nanoparticles‐Based Acetylcholinesterase Biosensor |
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| Authors: | Lin Zhang Linjuan Long Weiying Zhang Dan Du Yuehe Lin |
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| Institution: | 1. Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, 430079, P.?R. China tel/fax: +86‐27‐67867958;2. Pacific Northwest National Laboratory, Richland, Washington, 99352, USA |
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| Abstract: | Organophosphate (OP) and carbamate pesticides exert their toxicity via attacking the hydroxyl moiety of serine in the ‘active site’ of acetylcholinesterase (AChE). In this paper we developed a stable AChE biosensor based on self‐assembling AChE to graphene nanosheet (GN)‐gold nanoparticles (AuNPs) nanocomposite electrode for investigation of inhibition, reactivation and aging processes of different pesticides. It is confirmed that pesticides can inhibit AChE in a short time. OPs poisoning is treatable with oximes while carbarmates exposure is insensitive to oximes. The proposed electrochemical approach thus provides a new simple tool for comparison of pesticide sensitivity and guide of therapeutic intervention. |
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| Keywords: | Acetylcholinesterase biosensor Graphene‐Au nanocomposite Organophosphates Carbamates |
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