ISOLATION AND PURIFICATION OF ALKALOIDS FROM PLUMULA NELUMBINIS BY DOUBLE-COLUMN ADSORPTION CHROMOTOGRAPHY |
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| Authors: | ZHANG Jixiang GUO Jinsheng OU Lailiang |
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| Institution: | 1. Department of Chemistry, Langfang Normal College, Langfang Hebei 065000, China
2. The Key Laboratory of Bioactive Materials of Education of Ministry, College of Life Science, Nankai University, Tiajin 300071, China |
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| Abstract: | The performance of adsorption and separation for liensinine, isoliensinine and neferine was studied by double-column adsorption chromatography using macroporous adsorption and cation exchange resins. The alkaloid extract with 49.2% total contents by mass representing 10.6% liensinine, 10.6% isoliensinine and 28.0% neferine respectively was prepared by D72 cation exchange resins, in which most of the impurities were water-soluble alkaloids. Furthermore, the alkaloid extract with 82.6% total contents by mass containing 33.1%, 15.0% and 34.5% of the three adsorbates respectively was prepared by double-column adsorption chromatography using AKS-W macroporous adsorption and D72 cation exchange resins. As a result, the content of single and total alkaloids has been greatly increased by the double-column adsorption chromatography. |
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| Keywords: | Double-column adsorption chromatography Macroporous adsorption resin Cation exchange resin Alkaloids from plumula nelumbinis |
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