Bioluminescence-based imaging technique for pressure measurement in water |
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Authors: | Yasunori Watanabe Yasufumi Tanaka |
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Institution: | (1) Graduate School of Engineering, Hokkaido University, North 13 West 8, Sapporo 060 8628, Japan;(2) Construction Department, Tokyo Electric Power Company, Tokyo, Japan |
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Abstract: | The dinoflagellate Pyrocystis lunula emits light in response to water motion. We developed a new imaging technique for measuring pressure using plankton that
emits light in response to mechanical stimulation. The bioluminescence emitted by P. lunula was used to measure impact water pressure produced using weight-drop tests. The maximum mean luminescence intensity correlated
with the maximum impact pressure that the cells receive when the circadian and diurnal biological rhythms are appropriately
controlled. Thus, with appropriate calibration of experimentally determined parameters, the dynamic impact pressure can be
estimated by measuring the cell-flash distribution. Statistical features of the evolution of flash intensity and the probability
distribution during the impacting event, which are described by both biological and mechanical response parameters, are also
discussed in this paper. The practical applicability of this bioluminescence imaging technique is examined through a water
drop test. The maximum dynamic pressure, occurring at the impact of a water jet against a wall, was estimated from the flash
intensity of the dinoflagellate. |
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