High voltage capillary zone electrophoresis: Operating parameters effects on electroendosmotic flows and electrophoretic mobilities

Summary In High Voltage Capillary Zone Electrophoresis a field is applied across a narrow bore capillary filled with electrolyte solution. An electroendosmotic (EEO) flow is generated within this capillary which sweeps solutes along the tube. An absolute method of flow estimation is described, along with some operating parameter effects on the solute mobility. System parameters enabling flow direction reversal and a zero flow are described. The use of several capillaries simultaneously and the effect of pH on EEO flow rates are also shown. Effects of various operating parameters on solute resolution are also detailed.