Affinity partitioning of yeast and horse liver alcohol dehydrogenases in polyethylene glycol-dyes/dextran two-phase systems |
| |
Authors: | J. -H. J. Pesliakas V. D. Zutautas A. A. Glemza |
| |
Affiliation: | (1) ESP Fermentas , All-Union Research institute of Applied Enzymology, 232028 Vilnius, Lithuanian SSR, USSR |
| |
Abstract: | Summary Affinity partition of yeast and horse liver alcohol dehydrogenases (ADH) in two-phase systems containing polyethylene glycol (PEG)-dyes is most effective using Cu(II) complexes of Light resistant yellow 2KT and Red-violet 2KT. The effects of NaCl, NAD and chelating agents (imidazole and adenine) on the partitioning of ADH's were studied. It was shown that two-phase systems containing dyes in the top and bottom phases with different affinity to yeast ADH are promising for the extraction of enzyme from crude extract. |
| |
Keywords: | Affinity partition Alcohol dehydrogenases Influence of dye-polyethylene glycol derivatives Influence of NaCl, NAD, chelating agents |
本文献已被 SpringerLink 等数据库收录! |