Affinity partitioning of yeast and horse liver alcohol dehydrogenases in polyethylene glycol-dyes/dextran two-phase systems |
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| Authors: | J -H J Pesliakas V D Zutautas A A Glemza |
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| Institution: | (1) ESP  Fermentas , All-Union Research institute of Applied Enzymology, 232028 Vilnius, Lithuanian SSR, USSR |
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| Abstract: | Summary Affinity partition of yeast and horse liver alcohol dehydrogenases (ADH) in two-phase systems containing polyethylene glycol (PEG)-dyes is most effective using Cu(II) complexes of Light resistant yellow 2KT and Red-violet 2KT. The effects of NaCl, NAD and chelating agents (imidazole and adenine) on the partitioning of ADH's were studied. It was shown that two-phase systems containing dyes in the top and bottom phases with different affinity to yeast ADH are promising for the extraction of enzyme from crude extract. |
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| Keywords: | Affinity partition Alcohol dehydrogenases Influence of dye-polyethylene glycol derivatives Influence of NaCl NAD chelating agents |
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