糖尿病大鼠血清一氧化氮浓度的变化

目的 :观察不同病程糖尿病大鼠血清一氧化氮 (NO)浓度变化 ,探讨糖尿病大鼠血清NO变化的规律和意义。方法 :建立链脲佐菌素诱导的糖尿病大鼠模型 ,分别在第 2、7、12周 3个时期取血清 ,葡萄糖酶法测定血清葡萄糖 ,硝酸还原酶法测定血清NO含量。结果 :(1)血糖变化　对照组各时期血糖值维持在正常水平 ;糖尿病组大鼠各时期血糖均明显高于对照组 (P <0 0 5 )。 (2 )血清NO浓度变化　对照组各时期血清NO浓度无显著性差异 ;糖尿病组大鼠 2周时血清NO浓度明显高于对照组 (P <0 0 5 ) ;7周、12周时糖尿病组大鼠血清NO浓度恢复到正常水平 ,与对照组相比无显著差异 (P >0 0 5 )。结论 :NO是糖尿病发生的重要因子之一 ,但可能与血糖的调节无关。

Changes of serum nitric oxide concentration in diabetic rats

Aim: To study the changes of serum nitric oxide concentration in different course of diabetic rats. Methods: STZ-induced diabetic rats were made. At the end of 2, 7 and 12 weeks, serum glucose concentration was estimated by glucose enzyme method, and serum nitric oxide concentration was estimated by nitrate reductase method.Results: (1) Changes of serum glucose: serum glucose of control group remained normal level, but serum glucose of diabetic group increased significantly in each time(P<0.05). (2)Changes of serum nitric oxide: serum nitric oxide of control group remained normal level, The serum nitric oxide of diabetic group increased significantly in 2 weeks(P<0.05), then decreased to normal level in 7 and 12 weeks(P>0.05).Conclusion:Nitric oxide may be one of the important factors on diabeties development, but may not play a role in regulating the serum glucose.