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Automated solid-phase extraction for determination of amodiaquine, chloroquine and metabolites in capillary blood on sampling paper by liquid chromatography |
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| Authors: | N Lindegårdh M Forslund M D Green A Kaneko Y Bergqvist |
| Institution: | 1. Dalarna University College, 781 88, Borl?nge, Sweden 2. Department of Analytical Chemistry, Uppsala University, P.O. Box 531, 751 21, Uppsala, Sweden 3. Division of Parasitic Diseases National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Mailstop F-12, 30333, Atlanta, GA, USA 4. Department of International Affairs and Tropical Medicine, Tokyo Women's Medical University School of Medicine, Tokyo, Japan 5. Department of Clinical Chemistry, Falun Central Hospital, 791 82, Falun, Sweden |
| Abstract: | Summary A bioanalytical method using solid-phase extraction and high-performance liquid chromatography (LC) has been developed and validated for the determination of amodiaquine, chloroquine and their metabolites in 100 μL blood applied to sampling paper. The intra-assay precision for all analytes was <5% at 2000 nM, <7% at 750 nM and <10% at 100 nM. The interassay precision for all analytes was <4% at 2000 nM, <7% at 750 nM and <12% at 100 nM. The lower limit of quantitation was 100 nM for all analytes. The limit of detection was 30 nM in 100 μL venous blood applied to sampling paper. |
| Keywords: | Column liquid chromatography Ion-pair chromatography Solid-phase extraction Antimalarial drugs Capillary blood |
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